An interactive website detailing the Cre-loxP system used in this research: http://www.dnalc.org/resources/nobel/capecchi.html
An introduction to adult mouse brain anatomy:
1. Go to the Allen Brain Institute reference atlas
2. Launch the 'coronal' or 'sagittal' interactive atlas viewer.
3. Click on any one of the sections images/schematics.
4. In the search box on the left type in the name of a brain region of interest, e.g. striatum, cortex, etc. The selected brain region will be highlighted in purple.
5. Click on consecutive sections to examine the location, size and span of the selected brain region.
An introduction to the types of cells in the brain:
1. Go to the GENSAT gene expression nervous system atlas. This atlas contains images of various brain regions from transgenic mice where different cell types are labeled with GFP reporter protein. Based on the GFP expression, one can examine the morphology of the different cell types.
2. Select the Cell Showcase tab
3. Using the menu options on the left, examine the morphology and characteristics of the following cell types: astrocyte, microglia, oligodendrocyte, Bergmann glia.
4. Using the menu options on the left, examine the morphology and characteristics of the following cell types: pyramidal cell, granule neuron of the hippocampus, motor neuron, Purkinje cell.
5. What is the difference between the cell types in (iii.) and (iv.)?
6. Write down the differences in morphology you observed between the cell types in (3) and (4). How do those morphological differences relate to cell function?